All nUView precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light – taking you from analysis to results faster than any other gel on the market! With nUView technology, you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while achieving improved results.
Visualization of Proteins in SDS-PAGE nUView System
SDS-PAGE (Sodium Dodecyl Sulphate – Polyacrylamide Gel Electrophoresis) is possibly the most common and long-standing analytical tool used in protein chemistry. The method is used to separate proteins according to their electrophoretic mobility which in SDS-PAGE is a function of length of polypeptide chain or molecular weight due to the proteins in the samples having near identical charge per unit mass due to the binding of SDS. Visualization commonly uses textile dyes such as Coomassie Brilliant Blue (CBB). These and other approaches such as silver staining, fluorescent dyes and heavy metal binding are protracted methods of staining. Most of these methods utilize either expensive reagents, cause problems with waste disposal or require specialized imaging systems.
Application: Dry eye Screening
TearPro Advantages
- Very few tear samples are needed for load: 0.2ul tears
- Short test time: can see results in 3 hour-Use NuSep’s precast gel-see results in 2 mins after running the gels
- Full Picture of Whole tear proteome